PXD054718-1

PXD054718 is an original dataset announced via ProteomeXchange.

Title	Glycoprofile Comparison of SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines
Description	This study assessed the N-glycosylation profiles of a full-length trimeric spike protein expressed in either HEK or CHO cells. Glycopeptide analysis was performed using a tandem mass spectrometry workflow and BioPharma Finder™ using HEK and CHO glycan databases for protein characterisation. The results outline important differences in the variety and types of N-glycan generated by the two cell lines across the 22 known N-glycosylation sites of the spike protein. A notable increase in terminal sialylation, as well as the presence of the potentially immunogenic N-glycolylneuraminic acid at a functionally key N-glycosylation site was observed in the CHO derived spike protein. With the potential for the relatively vast and more complex CHO glycan repertoire (182 glycans relative to 39 human glycans) to produce functional implications with CHO expressed spike protein, this study adds valuable knowledge to aid Quality by Design approaches and enable Multi Attribute Monitoring of specific N-glycosylation sites for proteoform analyses. This can further inform antigen development with future variants, to devise updated diagnostic tests and therapeutic vaccine designs
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:10:19.312.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helen Wright
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 08:34:24	ID requested
⏵ 1	2025-09-14 16:10:20	announced

10.1007/s12033-024-01288-2;

Wright HL, Evans C, Jackson PJ, James DC, Tee KL, Wong TS, Dickman MJ, Pandhal J, Glycoprofile Comparison of the SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines. Mol Biotechnol, 67(9):3737-3752(2025) [pubmed]

submitter keyword: COVID-19, HEK Cells, MS-MS, SARS-CoV-2, N-Glycosylation, CHO Cells

Jagroop Pandhal
contact affiliation	School of Chemical, Materials and Biological Engineering; University of Sheffield.
contact email	j.pandhal@sheffield.ac.uk
lab head
Helen Wright
contact affiliation	University of Sheffield
contact email	helen.wright@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD054718

PRIDE project URI

• PRIDE
  1. PXD054718
     1. Label: PRIDE project
     2. Name: Glycoprofile Comparison of SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines