PXD054703-1

PXD054703 is an original dataset announced via ProteomeXchange.

Title	Identification of a FimA protein in a crude lysate SDS-PAGE gel of a P. gingivalis heme-uptake mutant
Description	Porphyromonas gingivalis is one of the main etiological agents responsible for initiating and progressing periodontal diseases in humans. P. gingivalis does not synthesize heme, and it uses host hemoproteins as a source of this molecule. The main heme uptake mechanism utilized by P. gingivalis is the Hmu system composed of six proteins where HmuY (hemophore-like protein) and HmuR (TonB-dependant outer membrane heme receptor) play a crucial role in heme supply. The second heme uptake mechanism is the Hus system composed of four proteins, with HusA (hemophore-like protein) and HusB (TonB-dependant outer membrane receptor), having a supporting role in heme uptake. Heme and iron starvation influence the P. gingivalis phenotype, therefore the aim was to determine the influence of deletion of HmuY and HmuR (ΔhmuYR strain) or HusA and HusB (ΔhusAB strain) encoding genes on P. gingivalis phenotype including the impact on protein production in response to the analyzed deletion. The mutant strains were constructed in wild-type A7436 P. gingivalis strain. Bacteria were grown in basal medium (BM) supplemented with 7.7 µM heme. After analysis of bacteria lysates with SDS-PAGE and CBB G-250 staining, in ΔhmuYR a protein (~45 kDa) was detected produced in higher amounts, than in the wild-type and the ΔhusAB strains. The gel band of interest, as well as its corresponding sections in the WT lanes were excised for bottom-up LC-MS analysis, and the protein was identified as FimA.
HostingRepository	PRIDE
AnnounceDate	2024-12-28
AnnouncementXML	Submission_2024-12-28_14:42:26.352.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054703
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Tracz
SpeciesList	scientific name: Porphyromonas gingivalis A7436; NCBI TaxID: 1403337;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 04:13:52	ID requested
⏵ 1	2024-12-28 14:42:26	announced

10.6019/PXD054703;

10.1038/S41598-024-82326-6;

submitter keyword: pili, SDS-PAGE, gel band analysis, pilus, bottom-up, heme-uptake mutant, MSE, hmuR,LC-MS, major fimbrium, FimA, hmuY, Porphyromonas gingivalis

Michal Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054703
     1. Label: PRIDE project
     2. Name: Identification of a FimA protein in a crude lysate SDS-PAGE gel of a P. gingivalis heme-uptake mutant