PXD054701-1

PXD054701 is an original dataset announced via ProteomeXchange.

Title	Interactome of chromatin-associated RNA Polymerase II from U2OS cells
Description	To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative mass spectrometry. U2OS cells expressing no HA-tagged protein were used for comparison.
HostingRepository	PRIDE
AnnounceDate	2024-12-20
AnnouncementXML	Submission_2024-12-20_06:03:01.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pranjali Bhandare
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 03:53:46	ID requested
⏵ 1	2024-12-20 06:03:01	announced

10.1093/nar/gkae1219;

Bhandare P, Narain A, Hofstetter J, Rummel T, Wenzel J, Sch, ü, lein-V, ö, lk C, Lamer S, Eilers U, Schlosser A, Eilers M, Erhard F, Wolf E, Phenotypic screens identify SCAF1 as critical activator of RNAPII elongation and global transcription. Nucleic Acids Res, ():(2024) [pubmed]

submitter keyword: Interactome, U2OS,RNAPII

Elmar Wolf
contact affiliation	Institute of Biochemistry, University of Kiel
contact email	elmar.wolf@biochem.uni-kiel.de
lab head
Pranjali Bhandare
contact affiliation	University of Kiel
contact email	pbhandare@biochem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD054701

PRIDE project URI

• PRIDE
  1. PXD054701
     1. Label: PRIDE project
     2. Name: Interactome of chromatin-associated RNA Polymerase II from U2OS cells