PXD054658-1
PXD054658 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An ERK biosensor identifies phosphatases regulating ERK pathway in tissue development and carcinoma cells |
| Description | Extracellular signal-regulated kinases (ERKs) play essential roles in development and are implicated in numerous diseases. Phosphatases act as key regulators of ERK activation. To obtain a view of the phosphorylation network regulating ERK activity, we describe a binary approach using mammalian cell culture and forward genetic screens in a model organism. We analyzed a spectrum of phosphatases in human cells with ERK-SPARK reporters. Further validation of selected components in Drosophila revealed a functional map of ERK pathway phosphatases. EYA family phosphatases were selected and characterized as novel and critical regulators of ERK signaling in vivo and in mammalian cells. Chemical screens using phosphatase inhibitors showed that pharmacological inhibition of Drosophila Eyes Absent Homologue 2 (EYA2) promotes the progression of ERK-dependent carcinoma. The kinase reporter enabled high-throughput screening of genetic and pharmacological regulators under physiological and pathological condition, an important approach for investigating the regulation of kinase signaling. |
| HostingRepository | iProX |
| AnnounceDate | 2024-08-07 |
| AnnouncementXML | Submission_2025-01-01_19:58:43.396.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yue Li |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-07 00:25:15 | ID requested | |
| ⏵ 1 | 2025-01-01 19:58:43 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: ERK signaling, biosensor, phosphatase, Drosophila |
Contact List
| Hai Huang | |
|---|---|
| contact affiliation | Zhejiang University |
| contact email | haihuang@zju.edu.cn |
| lab head | |
| Yue Li | |
| contact affiliation | Zhejiang University |
| contact email | liyue9104@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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