PXD054625-1
PXD054625 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative Proteomics Unveils Synergistic Effects of Combination drugs on Cytoskeleton Composition and Autophagy-Mediated Cell Death in Neuroblastoma |
| Description | Neuroblastoma, a prevalent and aggressive childhood cancer, faces a critical lack of effective treatment options. We systematically analyzed small-molecule perturbation gene expression data from the Library of Integrated Network-Based Cellular Signatures (LINCS), aiming to identify drugs with promising with potential for combination therapy. Following this predictive phase, we proceeded to validate our findings through a series of comprehensive assays. To achieve this, we employed an array of advanced techniques: quantitative proteomics with tandem mass tag labeling, western blotting, immunocytochemistry, and migration analysis. This multifaceted approach allowed us to gain a more profound understanding of the therapeutic potential and the cellular dynamics involved in treating neuroblastoma. Our research led us to hypothesize that a synergistic effect could be achieved in neuroblastoma treatment using a combination of two FDA-approved drugs: sirolimus and pyrvinium pamoate. Upon administering this combined therapy, we observed a significant reduction in cell viability, which was also accompanied by decreased mRNA expression. The effectiveness of this combination was further underscored by colony formation assays, which revealed a notably lower survival rate in neuroblastoma cell lines treated with the drug duo, as opposed to those treated with either drug in isolation. Our comprehensive proteomics analysis, encompassing the identification of 20,623 peptides and 3,415 proteins, shed light on the underlying biological processes affected by the therapy. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-09-08 |
| AnnouncementXML | Submission_2025-09-09_08:00:05.193.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hsueh-Fen Juan |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-06 01:15:01 | ID requested | |
| ⏵ 1 | 2025-09-09 08:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Neuroblastoma, Combination therapy, Sirolimus, Pyrvinium pamoate, Tandem mass tag |
Contact List
| Hsueh-Fen Juan | |
|---|---|
| lab head | |
| Hsueh-Fen Juan | |
| contact affiliation | Department of Life Science, National Taiwan University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003261/ |
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