PXD054611-1

PXD054611 is an original dataset announced via ProteomeXchange.

Title	RAD52 resolves transcription-replication conflicts to mitigate R-loop induced genome instability
Description	Collisions of the transcription and replication machineries on the same DNA strand can pose a significant threat to genomic stability. These collisions occur in part due to the formation of RNA-DNA hybrids termed R-loops, in which a newly transcribed RNA molecule hybridizes with the DNA template strand. This study investigated the role of RAD52, a known DNA repair factor, in preventing collisions by directing R-loop formation and resolution. We show that RAD52 deficiency increases R-loop accumulation, exacerbating collisions and resulting in elevated DNA damage. Furthermore, RAD52's ability to interact with the transcription machinery, coupled with its capacity to facilitate R-loop dissolution, highlights its role in preventing collisions. Lastly, we provide evidence of an increased mutational burden from double-strand breaks at conserved R-loop sites in human tumor samples, which is increased in tumors with low RAD52 expression. In summary, this study underscores the importance of RAD52 in orchestrating the balance between replication and transcription processes to prevent collisions and maintain genome stability.
HostingRepository	PRIDE
AnnounceDate	2024-08-25
AnnouncementXML	Submission_2024-08-24_19:42:49.276.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Manisha Jalan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-05 17:37:37	ID requested
⏵ 1	2024-08-24 19:42:49	announced

10.6019/PXD054611;

10.1038/S41467-024-51784-X;

submitter keyword: RAD52, IP-Mass Spec, Human Cells

Manisha Jalan
contact affiliation	Sloan Kettering Institute
contact email	jalanm@mskcc.org
lab head
Manisha Jalan
contact affiliation	Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center
contact email	jalanm@mskcc.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054611
     1. Label: PRIDE project
     2. Name: RAD52 resolves transcription-replication conflicts to mitigate R-loop induced genome instability