PXD054588-1

PXD054588 is an original dataset announced via ProteomeXchange.

Title	Tumour sampling conditions perturb the metabolic landscape of clear cell renal cell carcinoma
Description	Human isotopic tracer studies are fast becoming the gold standard model to study cancer metabolism in vivo. Analysed tissues are typically retrieved after surgical resection, which exposes them to a variable extent of warm ischaemia. Although standardised protocols are emerging, the effects of sampling conditions on the tissue metabolome remain understudied. Here, we perform a 13C-glucose study coupled with metabolomic, transcriptomic, and proteomic profiling in patients with clear cell renal cell carcinoma (ccRCC) to assess the metabolic profile of tissues sampled intraoperatively, when blood supply is intact, versus post-surgical resection, which exposes the tissue to ischaemia. We show that ischaemia significantly affects the metabolic landscape of ccRCC, masking some critical features, such as suppressed gluconeogenesis. Furthermore, in orthotopic ccRCC-derived xenografts, we identified that prolonged exposure to ischaemia disrupts the tissue metabolome stability. Therefore, minimising tissue ischaemia is
HostingRepository	PRIDE
AnnounceDate	2025-08-31
AnnouncementXML	Submission_2025-08-30_17:55:02.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von Kriegsheim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-05 03:09:47	ID requested
⏵ 1	2025-08-30 17:55:03	announced

Dataset with its publication pending

submitter keyword: 13C-glucose, metabolism, human isotopic tracers, clear cell renal cell carcinoma (ccRCC), in vivo, kidney cancer, ischaemia

Christian Frezza
contact affiliation	University of Cologne
contact email	christian.frezza@uni-koeln.de
lab head
Alex von Kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054588
     1. Label: PRIDE project
     2. Name: Tumour sampling conditions perturb the metabolic landscape of clear cell renal cell carcinoma