PXD054530-1

PXD054530 is an original dataset announced via ProteomeXchange.

Title	Identifying the phosphorylation sites on PNET1 protein in Arabidopsis.
Description	The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the essential transport of molecules between the nucleus and cytoplasm. Emerging evidence suggests its potential involvement in cell cycle regulation, but the specific roles and mechanisms of nucleoporins in this process remain largely uncharted. We previously found that PNET1 is a transmembrane nucleoporin and is expressed in actively dividing regions in Arabidopsis thaliana. Previous studies revealed that nucleoporins are phosphorylated during mitosis to facilitate the nuclear envelope breakdown process. In addition, we found that PNET1 extracted from callus tissues created a shifted band after running on Phos-tag gel, while this band was not observed when PNET1 is extracted from seedlings. We used LC-MS/MS to search the phosphorylation sites on PNET1 to further understand where exactly phosphorylation is happening and to create phospho-mimic and phospho-deficient versions of PNET1 later on. We detected more than 10 phosphorylation sites on PNET1.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:04:06.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-02 04:00:17	ID requested
⏵ 1	2025-08-03 16:04:07	announced

10.1038/s41477-025-01908-y;

Fang Y, Tang Y, Xie P, Hsieh K, Nam H, Jia M, Reyes AV, Liu Y, Xu S, Xu X, Gu Y, Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division. Nat Plants, 11(2):295-308(2025) [pubmed]

submitter keyword: Phosphorylation, Nuclear pore complex, immunoprecipitation, cell cycle, callus, LC-MS/MS

SHOULING XU
contact affiliation	Carnegie Mass Spectrometry Facility
contact email	sxu@carnegiescience.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD054530

PRIDE project URI

• PRIDE
  1. PXD054530
     1. Label: PRIDE project
     2. Name: Identifying the phosphorylation sites on PNET1 protein in Arabidopsis.