PXD054509-1

PXD054509 is an original dataset announced via ProteomeXchange.

Title	Dose-dependent proteome profiling of KRAS pathway inhibitors sheds new light on drug mechanism of action and KRAS signaling
Description	Kras is a molecular switch controlling the activity of the MAPK pathway. As such, it determines ERK activation state. In this study we identified many novel p-sites that were regulated upon KRAS inhibition. Many of these regulated p-sites were located within a SP/TP motif, the recognition motif of proline directed Kinases such as ERK. In this study we used parallel reaction monitoring (PRM) to determine the ability of ERK to phosphorylate synthetic peptides carrying a subset of these p-sites in a time-resolved manner. We identified that ERK was able to phosphorylate 10 of the tested sequences of which only one was phosphorylated on a mutated control peptide.
HostingRepository	PanoramaPublic
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-17_08:48:53.870.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Miriam Abele
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Acetyl; Phospho; Amidated; Oxidation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-01 15:31:20	ID requested
⏵ 1	2025-06-17 08:48:54	announced

Dataset with its publication pending

submitter keyword: KRAS, PRM, ERK

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, Freising, German
contact email	kuster@tum.de
lab head
Miriam Abele
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), TUM School of Life Sciences, Technical University of Munich, Freising, German
contact email	m.abele@tum.de
dataset submitter

Panorama Public dataset URI