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PXD054505-1

PXD054505 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranscription factor EB sets the stage for fate decisions of antigen-primed B cells in the absence and presence of co-stimulation
DescriptionLigation of the B cell antigen receptor (BCR) initiates humoral immunity. However, mere BCR signaling without appropriate co-stimulation commits B cells to death rather than to differentiation into immune effector cells. How BCR activation depletes potentially autoreactive B cells while simultaneously primes for receiving rescue and differentiation signals from cognate T lymphocytes remains unknown. Here, using a mass spectrometry-based proteomic approach to identify cytosolic/nuclear shuttling elements, we uncover transcription factor EB (TFEB) as a central BCR-controlled rheostat that drives activation-induced apoptosis, and concurrently, promotes the reception of co-stimulatory rescue signals by supporting B cell migration and antigen presentation. CD40 co-stimulation prevents TFEB-driven cell death, while enhancing and prolonging TFEB’s nuclear residency, which hallmarks antigenic experience also of memory B cells. In mice, TFEB shapes the transcriptional landscape of germinal center B cells. Within the germinal center, TFEB facilitates the dark zone entry of light-zone-residing centrocytes through regulation of chemokine receptors and, by balancing the expression of Bcl-2/BH3-only family members, integrates antigen-induced apoptosis with T cell-provided CD40 survival signals. Thus, TFEB reprograms antigen-primed germinal center B cells for cell fate decisions.
HostingRepositoryPRIDE
AnnounceDate2024-08-08
AnnouncementXMLSubmission_2024-08-08_03:41:57.893.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristof Lenz
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-01 09:27:21ID requested
12024-08-08 03:41:58announced
22024-10-22 06:52:59announced2024-10-22: Updated project metadata.
Publication List
10.1038/S41467-024-51166-3;
Keyword List
submitter keyword: B cell receptor, signal transduction,B cells, immune cell death, gene regulation in immune cells
Contact List
Christof Lenz
contact affiliationCore Facility Proteomics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany
contact emailchristof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliationMax Planck Institute for Biophysical Chemistry
contact emailchristof.lenz@mpibpc.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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