PXD054495-1

PXD054495 is an original dataset announced via ProteomeXchange.

Title	Striatum Proteomics from LacQ140 mutant Huntingtin lowering mouse model
Description	Lowering of mutant Huntingtin (mHtt) transcript and protein levels is considered a therapeutic strategy for people with Huntington’s disease (HD). However, the effects of mHtt lowering are incompletely understood. In this study, the proteomic changes in striatum upon Htt lowering were analyzed, using an inducible HD-knock-in LacQ140 mouse model with temporal control of mHtt-Q140 expression. The proteomic HD-disease signature in LacQ140 mouse striata was defined, using label-free quantification by liquid chromatography-mass spectrometry in data-independent acquisition mode. From the over 8000 protein groups identified in the striata, a disease signature of differentially regulated proteins in LacQ140 mice was identified, compared to wildtype control mice at 2-, 6-, 9- and 12-months of age. The effect of mHtt lowering on striatal proteomics was explored in LacQ140 mice, in which mHtt was lowered by repressing its transcription starting at an early or late time point, before or after onset of behavioral, molecular and aggregation phenotypes.
HostingRepository	PRIDE
AnnounceDate	2025-04-11
AnnouncementXML	Submission_2025-04-11_01:47:05.061.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Rolfs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-01 05:24:52	ID requested
⏵ 1	2025-04-11 01:47:06	announced

10.1016/j.nbd.2025.106906;

Marchionini DM, De Lombaerde S, van Rijswijk J, Zajicek F, Everix L, Miranda A, Aaltonen MJ, Kluger CM, Wild T, Kakoulidou A, Gundelach J, Fieblinger T, Fentz J, Rosinski J, Obenauer J, Greene JR, Liu L, Munoz-Sanjuan I, Verhoye M, Verhaeghe J, Bard J, Staelens S, Bertoglio D, Pharmacodynamic biomarkers responsive to mutant huntingtin lowering in a Huntington's disease mouse model. Neurobiol Dis, 209():106906(2025) [pubmed]

submitter keyword: Striata,Mouse, Huntington’s disease, LC-MS/MS

Christoph Schaab
contact affiliation	Evotec (München) GmbH Anna-Sigmund-Str. 5 82061 Neuried Germany
contact email	Christoph.Schaab@evotec.com
lab head
Frank Rolfs
contact affiliation	Evotec International GmbH Anna-Sigmund-Str. 5 82061 Neuried (Germany)
contact email	frank.rolfs@evotec.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054495

PRIDE project URI

• PRIDE
  1. PXD054495
     1. Label: PRIDE project
     2. Name: Striatum Proteomics from LacQ140 mutant Huntingtin lowering mouse model