PXD054441-1

PXD054441 is an original dataset announced via ProteomeXchange.

Title	Temporal (phospho-)proteomic profiling of neurotrophic receptor tyrosine kinase signalling in neuroblastoma cells
Description	Neurotrophic receptor tyrosine kinases, also known as Tropomyosin receptor kinases (TrkA, TrkB and TrkC), despite their homology, contribute to the clinical heterogeneity of childhood cancer neuroblastoma. TrkA expression is associated with low-stage disease and is often seen with spontaneous tumour regression. Conversely, TrkB is present in unfavourable neuroblastomas that often harbour amplification of the MYCN oncogene. The role of TrkC is less clearly defined, although some studies suggest its association with a favourable outcome. Understanding the differences in the activity of Trk receptors that drive divergent clinical phenotypes as well as the influence of MYCN amplification on downstream Trk receptor signalling remain poorly understood. Here, we present a comprehensive label-free mass spectrometry-based total proteomics and phosphoproteomics dataset where we identified and quantified 4,907 proteins, 16,744 phosphosites and 5,084 phosphoproteins, derived from NGF/BDNF/NT-3 treated TrkA/B/C-overexpressing neuroblastoma cells with differential MYCN status. Analysing our dataset offers valuable insights into TrkA/B/C receptor signalling in neuroblastoma and its modulation by MYCN status; and holds potential for advancing therapeutic strategies in this challenging childhood cancer.
HostingRepository	PRIDE
AnnounceDate	2024-08-29
AnnouncementXML	Submission_2024-08-29_11:30:10.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kieran Wynne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-31 06:10:23	ID requested
⏵ 1	2024-08-29 11:30:10	announced

Dataset with its publication pending

submitter keyword: neuroblastoma, Trk, phosphoproteome,Proteome

Melinda Halasz
contact affiliation	Assistant Professor in Pathology Group Leader at Systems Biology Ireland & Conway FellowUCDSchool of Medicine, University College Dublin
contact email	melinda.halasz@ucd.ie
lab head
Kieran Wynne
contact affiliation	University College Dublin
contact email	kieran.wynne1@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054441
     1. Label: PRIDE project
     2. Name: Temporal (phospho-)proteomic profiling of neurotrophic receptor tyrosine kinase signalling in neuroblastoma cells