PXD054402-1

PXD054402 is an original dataset announced via ProteomeXchange.

Title	LC-MS3 analysis of Herc1-knockout mouse MLL/AF9 acute myeloid leukemia cells
Description	Our CRISPR-based pharmacogenomic screening identified the E3 ubiquitin ligase Herc1 as a key modulator of sensitivity to cytarabine (Ara-C) chemotherapy in the MLL/AF9 (MA) mouse AML model both in vitro and in vivo. To identify the intracellular proteins whose abundance is modulated by Herc1, we performed comparative LC-MS3 analysis of in vitro-cultured MA mouse AML cells that were transduced with either a small-guide RNA targeting Herc1 (i.e., CRISPR-mediated Herc1-knockout) or a control non-targeting small-guide RNA, at steady state or after brief exposure to Ara-C (200 nMol for 6 hours).
HostingRepository	PRIDE
AnnounceDate	2024-08-08
AnnouncementXML	Submission_2024-08-08_02:28:00.955.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	François Mercier
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 09:20:23	ID requested
⏵ 1	2024-08-08 02:28:01	announced
2	2024-10-22 06:52:47	announced	2024-10-22: Updated project metadata.

10.1182/bloodadvances.2023011540;

Jankovic M, Poon WWL, Gonzales-Losada C, Vazquez GG, Sharif-Askari B, Ding Y, Craplet-Desombre C, Ilie A, Shi J, Wang Y, Jayavelu AK, Orthwein A, Mercier F É, The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia. Blood Adv, 8(20):5315-5329(2024) [pubmed]

submitter keyword: Herc1, mouse, LC-MS3,Acute myeloid leukemia

Francois Mercier
contact affiliation	Lady Davis Institue for Medical Research, McGill University, Canada Department of Medicine, McGill University, Canada
contact email	francois.mercier@mcgill.ca
lab head
François Mercier
contact affiliation	McGill University
contact email	francois.mercier@mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD054402

PRIDE project URI

• PRIDE
  1. PXD054402
     1. Label: PRIDE project
     2. Name: LC-MS3 analysis of Herc1-knockout mouse MLL/AF9 acute myeloid leukemia cells