PXD054389-1

PXD054389 is an original dataset announced via ProteomeXchange.

Title	Enhancing Uterine Receptivity for Embryo Implantation through Controlled Collagenase Intervention
Description	Ineffective endometrial matrix remodeling, a key factor in infertility, impedes embryo implantation in the uterine wall. Our study reveals the cellular and molecular impact of human collagenase-1 administration in mouse uteri, demonstrating enhanced embryo implantation rates. Collagenase-1 promotes remodeling of the endometrial extracellular matrix (ECM), degrading collagen fibers and proteoglycans. This process releases matrix-bound bioactive factors, (e.g. VEGF, decorin), facilitating vascular permeability and angiogenesis. Collagenase-1 elevates embryo implantation regulators, including NK cell infiltration and the key cytokine LIF. Remarkably, uterine tissue maintains structural integrity despite reduced endometrial collagen fiber tension. In-utero collagenase-1 application rescues implantation in the heat stress and embryo transfer models, known for low implantation rates. Importantly, ex-vivo exposure of human uterine tissue to collagenase-1 induces collagen de-tensioning and VEGF release, mirroring remodeling observed in mice. Our research highlights collagenase potential to induce and orchestrate cellular and molecular processes enhancing uterine receptivity for effective embryo implantation. This innovative approach underscores ECM remodeling mechanisms critical for embryo implantation
HostingRepository	PRIDE
AnnounceDate	2024-08-13
AnnouncementXML	Submission_2024-08-13_03:39:32.154.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054389
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Idan Adir
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 06:15:24	ID requested
⏵ 1	2024-08-13 03:39:32	announced
2	2024-10-22 06:54:46	announced	2024-10-22: Updated project metadata.

10.26508/LSA.202402656;

10.6019/PXD054389;

submitter keyword: embryo, implantation, matrix proteolysis, collagen remodeling, collagenase-1,ECM

Irit Sagi
contact affiliation	Department of Immunology and Regenerative Biology The Weizmann Institute of Science
contact email	irit.sagi@weizmann.ac.il
lab head
Idan Adir
contact affiliation	The Weizmann Institute for Science
contact email	idanadir@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054389
     1. Label: PRIDE project
     2. Name: Enhancing Uterine Receptivity for Embryo Implantation through Controlled Collagenase Intervention