PXD054387-1

PXD054387 is an original dataset announced via ProteomeXchange.

Title	Elucidation of the substrate specificity of the ABC transporter Tba uncovers an unsuspected role in the biosynthesis of glycopeptide antibiotics
Description	In this project, the proximal proteins of the balhimycin transporter Tba in A. balhimycina were investigated in order to elucidate potential interaction partners. Tba is an ABC transporter, which is co-encoded with biosynthetic enzymes in the biosynthetic gene cluster of the glycopeptide antibiotic (GPA) balhimycin. Little is known about the interactions and regulatory roles of GPA exporters. Therefore, a proximity biotinylation approach was used to label proximal proteins prior to identification by mass spectrometry. Tba was expressed as a fusion protein with the promiscuous biotin ligase TurboID.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_22:33:13.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Amycolatopsis balhimycina; NCBI TaxID: 208443;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 06:01:07	ID requested
⏵ 1	2025-05-06 22:33:14	announced

10.1016/j.isci.2025.112135;

Gericke N, Beqaj D, Kronenberger T, Kulik A, Gavriilidou A, Franz-Wachtel M, Schoppmeier U, Harbig T, Rapp J, Grin I, Ziemert N, Link H, Nieselt K, Macek B, Wohlleben W, Stegmann E, Wagner S, Unveiling the substrate specificity of the ABC transporter Tba and its role in glycopeptide biosynthesis. iScience, 28(4):112135(2025) [pubmed]

submitter keyword: Amycolatopsis balhimycina, Glycopeptide antibiotics, Balhimycin, ABC Transporter

Samuel Wagner
contact affiliation	Cellular and Molecular Microbiology Interfaculty Institute of Microbiology and Infection Medicine (IMIT) University of Tübingen Elfriede-Aulhorn-Str. 6 72076 Tübingen, Germany
contact email	Samuel.Wagner@med.uni-tuebingen.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054387

PRIDE project URI

• PRIDE
  1. PXD054387
     1. Label: PRIDE project
     2. Name: Elucidation of the substrate specificity of the ABC transporter Tba uncovers an unsuspected role in the biosynthesis of glycopeptide antibiotics