PXD054373-1

PXD054373 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of differentially expressed proteins by p53 status and cholesterol concentration of culture medium in on-top 3D culture using MDA-MB-231
Description	Tumor cells had p53 knocked down by CRISPR/Cas9. In addition, Doxycycline-induced p53WT or p53R280K was incorporated into the p53KO cells by the Tet-on system. Furthermore, we created a high cholesterol condition in that Cholesterol was added to the culture medium while p53R280K was being expressed, and a low cholesterol condition in which Cholesterol was reduced by reducing serum.　The cells were cultured for 7 days in on-top 3D with respect to these four conditions. Whole cell lysates were processed for non-targeted proteomic analysis.
HostingRepository	jPOST
AnnounceDate	2025-09-14
AnnouncementXML	Submission_2025-09-13_08:00:05.550.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yusuke Kawashima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 01:20:42	ID requested
⏵ 1	2025-09-13 08:00:05	announced

Dataset with its publication pending

submitter keyword: Breast Cancer, p53, Cholesterol, on-top 3D, MDA-MB-231

Tomoaki Tanaka
lab head
Yusuke Kawashima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

jPOST dataset URI

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