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PXD054359-1

PXD054359 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLoss of ribosomal RNA methyltransferase Nsun5 Leads to Hypomyelination and Reduced Sleep Amount
DescriptionSleep is regulated by intricate homeostatic and circadian systems and is influenced by genetic, environmental, and lifestyle factors. However, the roles of the epitranscriptome in sleep-wake regulation remain to be clarified. Here, we find that mice lacking the rRNA methyltransferase Nsun5 exhibit prolonged wakefulness. The absence of Nsun5 decreases 28S rRNA m5C levels, impairing the translational efficiency of nascent proteins associated with myelination, microtubule-based movement, and ribosome binding. This leads to a decreased number of oligodendrocytes and hypomyelination. These deficits result in increased excitability, particularly within the cortex. Notably, restoring myelination properties with clemastine or blocking the abnormally exposed voltage-gated potassium channels rescues the sleep phenotype, suggesting that reduced sleep is associated with hypomyelination. These findings highlight the significant role of epigenetic modifications in sleep regulation and offer broad mechanistic and therapeutic implications for brain disorders involving both myelin and sleep disturbances.
HostingRepositoryiProX
AnnounceDate2024-07-29
AnnouncementXMLSubmission_2024-07-29_22:35:15.484.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeipei Chen
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-29 22:33:01ID requested
12024-07-29 22:35:15announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: sleep, Nsun5, RNA methylation modification, myelin sheath
Contact List
Zhili Huang
contact affiliationFudan University
contact emailhuangzl@fudan.edu.cn
lab head
Peipei Chen
contact affiliationFudan University
contact emailchen930513@163.com
dataset submitter
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