PXD054339 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | N-terminomics and proteomics analysis of Calpain-2 reveal key proteolytic processing of metabolic and cell adhesion proteins |
| Description | Aberrant levels of the cysteine protease Calpain-2 have been linked to neurodegeneration, inflammation, and cancer, yet our understanding of this protease and its substrates remains limited. Systematic studies to identify Calpain-2 substrates have been largely confined to peptide libraries or in vitro studies, which fail to represent physiological cellular conditions and physiologically relevant substrates. In addition to Calpain-2, Calpain-1 is also ubiquitously present in cells, making it challenging to understand the distinct contributions of Calpain-1 and Calpain-2 in cells and the difference between their substrates’ repertoires. We used a genetic approach to knockout Calpain-2 in the THP-1 human monocyte-like cells, followed by proteomic and N-terminomic/TAILS mass spectrometry approaches to identify Calpain-2 substrates. We identified 51 new putative Calpain-2 substrates. The direct cleavage of selected substrates by Calpain-2 was confirmed using in vitro assays. Metabolomics was performed and identified a role for Calpain-2 in the regulation of pyrimidine and glutathione metabolism. Our unbiased and quantitative mass spectrometry analytical pipeline provides new evidence on the physiological functions of Calpain-2 and its newly identified substrates in THP-1 cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-04-25 |
| AnnouncementXML | Submission_2025-04-25_12:06:03.161.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Luiz Gustavo de Almeida |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-07-29 05:22:25 | ID requested | |
| ⏵ 1 | 2025-04-25 12:06:03 | announced | |
Publication List
Keyword List
| submitter keyword: Metabolism,Calpain-2, Protease, Proteomics, N-terminomics, THP-1 monocyte |
Contact List
| Antoine Dufour |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada; Department of Physiology and Pharmacology, University of Calgary, Alberta, Canada; The Hotchkiss Brain Institute, University of Calgary, Alberta, Canada; McCaig Institute for Bone and Joint Health, University of Calgary, Alberta, Canada |
| contact email | antoine.dufour@ucalgary.ca |
| lab head | |
| Luiz Gustavo de Almeida |
|---|
| contact affiliation | University of Calgary |
| contact email | luizgustavo.biotec@hotmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054339 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054339
- Label: PRIDE project
- Name: N-terminomics and proteomics analysis of Calpain-2 reveal key proteolytic processing of metabolic and cell adhesion proteins
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