PXD054332-1

PXD054332 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The changes in HNSCC cell lines (e.g., CAL-27 cetuximabR) after being treated with Cetuximab (500 ug/ml) for at least 2 months. We used LC-MS/MS to identify the main acetylation sites in resistance cell lines. In this study, it was found that the acetylation of STAT1 might hinder the downstream signaling of IFN-γ
Description	Recent clinical trials revealed that prior use of Cetuximab may interfere with ICB efficacy. In this study, STAT1 protein levels changed after continued Cetuximab treatment (500 ug/ml). We used LC-MS/MS to compare the acetylation sites in wild-type and resistant cells. We proposed that the acetylation of STAT1 may impair IFN-γ response.
HostingRepository	PRIDE
AnnounceDate	2025-12-29
AnnouncementXML	Submission_2025-12-28_16:05:06.886.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054332
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Po-Hsien Chiu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-29 01:19:57	ID requested
⏵ 1	2025-12-28 16:05:07	announced

Publication List

10.1016/j.xcrm.2025.102448;
Chiu PH, Lai KC, Wang HL, Chang YW, Wu WC, Chen TH, Tsai YS, Song JH, Sun NY, Chau GY, Fang WL, Chen JP, Wang HM, Huang HC, Hsieh MC, Hua CH, Lien MY, Chang YF, Wang HC, Chien CY, Huang TL, Wang CC, Liu YC, Chen JP, Lu WC, Yiu CY, Lin CL, Lou PJ, Chu PY, Wang SC, Hung MC, Yang MH, Therapeutic stress triggers tumor STAT1 acetylation to disarm immunotherapy. Cell Rep Med, 6(11):102448(2025) [pubmed]
10.6019/PXD054332;

10.1016/j.xcrm.2025.102448;

Chiu PH, Lai KC, Wang HL, Chang YW, Wu WC, Chen TH, Tsai YS, Song JH, Sun NY, Chau GY, Fang WL, Chen JP, Wang HM, Huang HC, Hsieh MC, Hua CH, Lien MY, Chang YF, Wang HC, Chien CY, Huang TL, Wang CC, Liu YC, Chen JP, Lu WC, Yiu CY, Lin CL, Lou PJ, Chu PY, Wang SC, Hung MC, Yang MH, Therapeutic stress triggers tumor STAT1 acetylation to disarm immunotherapy. Cell Rep Med, 6(11):102448(2025) [pubmed]

10.6019/PXD054332;

Keyword List

submitter keyword: Human, LC-MSMS, HNSCC, Cetuximab resistance, Acetylation

submitter keyword: Human, LC-MSMS, HNSCC, Cetuximab resistance, Acetylation

Contact List

Muh-Hwa Yang
contact affiliation	Institute of Clinical Medicine in National Yang-Ming Chiao Tung University, Taiwan (Muh-Hwa Yang Lab)
contact email	mhyang2@nycu.edu.tw
lab head
Po-Hsien Chiu
contact affiliation	National Yang Ming Chiao Tung University
contact email	ck901396@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054332
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054332

PRIDE project URI

Repository Record List

[ + ]