PXD054299-1

PXD054299 is an original dataset announced via ProteomeXchange.

Title	The 19S RP-associated deubiquitylation downstream of AP-3β disassembles heat-induced stress granules in plants
Description	To survive under adverse conditions, plants form stress granules (SGs) to temporally store mRNA and halt translation as a primary response. Dysregulation in SG disassembly can have detrimental effects on plant survival after stress release, yet the underlying mechanism remains poorly understood in plants. Using Arabidopsis as a model system, we demonstrated that the AP-3 subunit AP-3β participates in heat response independently of its conventional role in vacuolar transport. We also discovered that AP-3β serves as an adaptor to recruit the 19S regulatory particle (RP) of the proteasome to SGs upon heat induction. Notably, the 19S RP promotes SG disassembly through RP-associated deubiquitylation, independent of its proteolytic activity. This deubiquitylation process of SG components is crucial for translation reinitiation and growth recovery after heat release. Our findings shed light on the non-proteolytic function of the 19S RP in regulating SGs dynamics and provide insights into a non-degradation mechanism for cellular adaptation to environmental stresses
HostingRepository	PRIDE
AnnounceDate	2025-01-24
AnnouncementXML	Submission_2025-01-23_21:56:17.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Pang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-27 20:20:38	ID requested
⏵ 1	2025-01-23 21:56:17	announced

Dataset with its publication pending

submitter keyword: stress granules (SGs)

AP-3β

19S RP

deubiquitylation

non-proteolytic function

Ruixi Li
contact affiliation	Shenzhen Key Laboratory of Plant Genetic Engineering and Molecular Design, Institute of Plant and Food Science, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
contact email	lirx@sustech.edu.cn
lab head
Lei Pang
contact affiliation	Shenzhen Key Laboratory of Plant Genetic Engineering and Molecular Design, Institute of Plant and Food Science, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
contact email	pangl@sustech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD054299

PRIDE project URI

• PRIDE
  1. PXD054299
     1. Label: PRIDE project
     2. Name: The 19S RP-associated deubiquitylation downstream of AP-3β disassembles heat-induced stress granules in plants