<<< Full experiment listing

PXD054283-1

PXD054283 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUrine protein composition of common bottlenose dolphins (Tursiops truncatus) in the Gulf of Mexico, USA
DescriptionUrinary proteins provide valuable insights into renal health, with implications spanning human and domestic animal veterinary medical research. However, the field of marine mammal medicine lacks comprehensive studies on urine protein composition. This research aimed to fill this gap by 1) selecting an optimal search strategy that yields the highest number of protein families in the bottlenose dolphin urine based on post-translational modifications (PTMs), 2) describing the urine proteome of wild bottlenose dolphins (Tursiops truncatus) in the Gulf of the Mexico, USA, considering sex (females vs. males), site (Barataria Bay, LA vs. Sarasota Bay, FL), and comparing with California sea lions (Zalophus californianus). Ten urine samples (Barataria Bay, LA: N= 6; Sarasota Bay, FL: N=4) collected during 2023 catch-and-release heath assessment were proteolytically digested and analyzed using an UltiMate 3000 Nano LC and Fusion Lumos Orbitrap mass spectrometer. A 2-step search strategy, incorporating dehydroalanine formation and semi-trypsin on the list of unassigned spectra, significantly increased the number of identified protein families by an average of 6.2% compared to the 1-step strategy (P < 0.001, t = -8.32). The top 30 proteins in bottlenose dolphin urine were ranked according to an exponentially modified protein abundance index for comparison based on sex, site, and species. There were no significant differences in urine proteins between sexes or sites (padj > 0.05), although there was sperm contribution in two of the male bottlenose dolphin urine samples. Two putative antimicrobial proteins (cathelicidin and lysozyme) were identified and found to be abundant in bottlenose dolphin urine, similar to California sea lions. The study also identified 27 potential markers of acute kidney injury and 12 regulators of kidney stone formation. This study established a reference database of urinary proteins from bottlenose dolphins, aiding future research in monitoring and evaluating renal health in marine mammals.
HostingRepositoryPRIDE
AnnounceDate2024-08-01
AnnouncementXMLSubmission_2024-08-01_08:00:15.887.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD054283
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBenjamin Neely
SpeciesList scientific name: Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus); NCBI TaxID: 9739;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-26 10:12:11ID requested
12024-08-01 08:00:16announced
Publication List
10.6019/PXD054283;
Keyword List
submitter keyword: LC-MSMS, urine,common bottlenose dolphin
Contact List
Kelly N. Cusick, Alison M. Bland, Michael G. Janech, Benjamin A.Neely
contact affiliationNational Instiute of Standards and Technology, Charleston, SC, USA
contact emailbenjamin.neely@nist.gov
lab head
Benjamin Neely
contact affiliationNational Institute of Standards and Technology
contact emailbenjamin.neely@nist.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD054283
PRIDE project URI
Repository Record List
[ + ]