PXD054265-1
PXD054265 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HRMS1 DIA and DDA of Lyso-IP inputs from mouse gut epithelium and stem cells |
| Description | These mouse gut lysates contained three sets, with Set A including 21 DDA experiments and 21 DIA experiments, Set C including 24 DDA and 24 DIA experiments, and Set G including 27 DDA RAWs and 26 DIA RAWs (one of the Set G DDA experiments was run twice). Experiment Set A was intended to compare lysosomal compositions among ad libitum feeding, 24h fasted, and 24h fasted and re-fed conditions. Experiment Set C compared lysosomal compositions between germ-free and conventional microbiome states. Experiment Set G compared ad libitum and 24h fasted conditions within stem cells. In each case, these RAWs represent the inputs to lyso-IP, not the lysosomally-enriched samples that are produced from lyso-IP. Sample preparation was performed using the S-TRAP method, with MMTS (methyl methanethiosulfonate) alkalyting free Cys side chains and trypsin digesting C-terminal to lysines and arginines. DDA experiments collected spectra for all 120 minutes of a two hour run, while DIA experiments using the high resolution MS1 (HRMS1) technique collected scans at two different FAIMS compensation voltages for 75 minutes during each 105 minute run, with scan events ending when the B solvent of the gradient reached 45%. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-09-10 |
| AnnouncementXML | Submission_2024-09-10_03:03:53.576.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | David L. Tabb |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | L-cysteine methyl disulfide; Oxidation |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-26 01:41:39 | ID requested | |
| ⏵ 1 | 2024-09-10 03:03:53 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Lyso-IP, HRMS1 |
Contact List
| Omer Yilmaz | |
|---|---|
| contact affiliation | Massachusetts Institute of Technology |
| contact email | ohyilmaz@mit.edu |
| lab head | |
| John P. LaCava | |
| contact affiliation | University of Groningen |
| contact email | jlacava@rockefeller.edu |
| lab head | |
| David L. Tabb | |
| contact affiliation | University of Groningen |
| contact email | d.l.tabb@rug.nl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095444/ |
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