PXD054247-1

PXD054247 is an original dataset announced via ProteomeXchange.

Title	Analysis of insulin-regulated phosphorylation of AFF4 with Akti or S6ki and S6K invitro kinase
Description	Aff4 was recently identified as an insulin-regulated phosphoprotein. To investigate upstream kinases, we analysed immunoprecipitated Aff4 treated with our without insulin and treated with either an Akti or a P70S6Ki. These results showed that AFF4 is dependent on P70S6K and therefore an in vitro kinase assay was performed to validate direct phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2025-12-16
AnnouncementXML	Submission_2025-12-15_21:21:40.535.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-25 14:11:10	ID requested
⏵ 1	2025-12-15 21:21:41	announced

Dataset with its publication pending

submitter keyword: Akt, P70S6K, phosphorylation,AFF4

Benjamin Parker
contact affiliation	The Univeristy of Melbourne
contact email	ben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054247

PRIDE project URI

• PRIDE
  1. PXD054247
     1. Label: PRIDE project
     2. Name: Analysis of insulin-regulated phosphorylation of AFF4 with Akti or S6ki and S6K invitro kinase