PXD054245-1

PXD054245 is an original dataset announced via ProteomeXchange.

Title	LRP6-BirA proximity labelling proteomics comparing wildtype and AP2 binding motif mutant (YYYF) LRP6 in FlpIn T-REx cells
Description	Here we generated tetracycline inducible LRP6-BirA fusion lines in T-REx FlpIn cell lines for BioID proximity labelling proteomics experiments. We compare wt and mutant (YYYF>A mutant; mutates AP2 binding motifs to alanine) LRP6 binding partners following 12hrs Biotin labelling and Wnt3a treatment.
HostingRepository	PRIDE
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-30_01:59:31.361.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina Franco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-25 11:28:04	ID requested
⏵ 1	2025-04-30 01:59:31	announced

Dataset with its publication pending

submitter keyword: Wnt, LRP6,FlpIn HEK293T, BioID

Mariann Bienz
contact affiliation	MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus CB2 0QH Cambridge UK
contact email	mb2@mrc-lmb.cam.ac.uk
lab head
Catarina Franco
contact affiliation	MRC Laboratory of Molecular Biology
contact email	cffranco@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054245
     1. Label: PRIDE project
     2. Name: LRP6-BirA proximity labelling proteomics comparing wildtype and AP2 binding motif mutant (YYYF) LRP6 in FlpIn T-REx cells