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PXD054241-1

PXD054241 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBioID study of the PHB2 interactome in Schwann cells and how it changes depending on neuronal signals
DescriptionSchwann cells are critical for the proper development and function of the peripheral nervous system, where they form a collaborative relationship with axons. Past studies highlighted that a pair of proteins called the prohibitins play major roles in Schwann cell biology. Prohibitins are ubiquitously expressed and versatile proteins. We have previously shown that while prohibitins play a crucial role in Schwann cell mitochondria for long-term myelin maintenance and axon health, they may also be present at the Schwann cell-axon interface during development. The current data set uses BioID to identify a pool of candidate PHB2 interactors in Schwann cells and explores how the PHB2 interactome changes depending on neuronal signals. We expressed a PHB2-turboID fusion construct in primary rat Schwann cells; turboID is a 35 kDa engineered biotin ligase that rapidly biotinylates proximal proteins. Thus, proteins interacting with PHB2-turboID within an approximately 10 nm radius are tagged with biotin. As a control, primary rat Schwann cells were transfected with an unfused turboID construct (Con-turboID). Schwann cells expressing Con-turboID or PHB2-turboID were plated alone or onto primary rat DRG neurons, in the presence or absence of biotin. After 2 hours, proteins were harvested from the cultures and biotinylated proteins were purified using streptavidin-affinity purification (AP). We then used liquid chromatography-mass spectrometry (LC-MS) to identify the PHB2-turboID interactors (the biotinylated proteins) in our purified pool. The PHB2 interactors identified here, especially those which increase or decrease interaction with PHB2-turboID in the presence of neurons, may play a role in prohibitin-associated Schwann cell-axon communication.
HostingRepositoryPRIDE
AnnounceDate2024-09-02
AnnouncementXMLSubmission_2024-09-02_09:16:46.553.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShuo Qian
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-25 09:09:00ID requested
12024-09-02 09:16:46announced
22024-10-22 06:57:39announced2024-10-22: Updated project metadata.
Publication List
10.1002/GLIA.24610;
Keyword List
submitter keyword: Interactome
Contact List
Jun Qu
contact affiliationDepartment of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY, USA
contact emailjunqu@buffalo.edu
lab head
Shuo Qian
contact affiliationDepartment of Cell Stress Biology, Roswell Park Comprehensive Cancer Center
contact emailsqian@buffalo.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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