PXD054205-1

PXD054205 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics and proteomics of insulin signaling in aged mouse bone
Description	We compared the in vivo insulin signaling response in bone tissue of 10- verses 73-week old C57BL/6J male mice (Figure 1A). Saline or insulin (2.5 mU/kg) was interperitoneally injected and mice sacrificed after 20 min. Tibia bone was rapidly dissected and flushed with ice-cold PBS within 30 sec to remove marrow and quench phosphorylation before being snap frozen in liquid nitrogen. Protein was extracted from powdered bone tissue and digested with trypsin and LysC followed by multiplexed stable isotope labelling with Tandem Mass Tags (TMT). Enriched phosphopeptides were analyzed by multi-dimensional ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (2DLC-MS/MS). In parallel, we also quantified non-enriched peptides to analyze relative proteome abundance differences. All mice were kept on a standard chow diet.
HostingRepository	PRIDE
AnnounceDate	2025-12-16
AnnouncementXML	Submission_2025-12-15_22:13:26.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-24 20:55:17	ID requested
⏵ 1	2025-12-15 22:13:26	announced

Dataset with its publication pending

submitter keyword: insulin signaling,bone, phosphoproteomics

Benjmain Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054205
     1. Label: PRIDE project
     2. Name: Phosphoproteomics and proteomics of insulin signaling in aged mouse bone