PXD054202-1

PXD054202 is an original dataset announced via ProteomeXchange.

Title	SLC17 transporters mediate renal excretion of Lac-Phe in mice and humans
Description	N-lactoyl-phenylalanine (Lac-Phe) is a lactate-derived metabolite that suppresses food intake and body weight. Little is known about the mechanisms that mediate Lac-Phe transport across cell membranes. Here we identify SLC17A1 and SLC17A3, two kidney-restricted plasma membrane-localized solute carriers, as physiologic urine Lac-Phe transporters. In cell culture, SLC17A1/3 exhibit high Lac-Phe efflux activity. In humans, levels of Lac-Phe in urine exhibit a strong genetic association with the SLC17A1-4 locus. Urine Lac-Phe levels are also increased following a Wingate sprint test. In mice, genetic ablation of either SLC17A1 or SLC17A3 reduces urine Lac-Phe levels. Despite these differences, both knockout strains have normal blood Lac-Phe and body weights, demonstrating SLC17-dependent de-coupling of urine and plasma Lac-Phe pools. Together, these data establish SLC17A1/3 family members as the physiologic urine transporters for Lac-Phe and uncover a biochemical pathway for the renal excretion of this signaling metabolite. Our data do not exclude the involvement of other transporters in mediating Lac-Phe transport.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:50:41.700.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054202
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Veronica Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-24 20:06:30	ID requested
⏵ 1	2024-10-17 02:50:42	announced

10.1038/s41467-024-51174-3;

Li VL, Xiao S, Schlosser P, Scherer N, Wiggenhorn AL, Spaas J, Tung AS, Karoly ED, K, ö, ttgen A, Long JZ, SLC17A1/3 transporters mediate renal excretion of Lac-Phe in mice and humans. Nat Commun, 15(1):6895(2024) [pubmed]

10.6019/PXD054202;

submitter keyword: Kidney,Lac-Phe, Transporter, Urine

Jonathan Zhong Long
contact affiliation	Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
contact email	jzlong@stanford.edu
lab head
Veronica Li
contact affiliation	Stanford University
contact email	ver0nica@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054202

PRIDE project URI

• PRIDE
  1. PXD054202
     1. Label: PRIDE project
     2. Name: SLC17 transporters mediate renal excretion of Lac-Phe in mice and humans