PXD054183 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nuclear release of eIF1 globally increases stringency of start codon selection to preserve mitotic arrest physiology |
Description | Regulated start codon selection has the potential to reshape the proteome through the differential production of uORFs, canonical proteins, and alternative translational isoforms. However, conditions under which start codon selection is altered remain poorly defined. Here, using transcriptome-wide translation initiation site profiling, we reveal a global increase in the stringency of start codon selection during mammalian mitosis. Low-efficiency initiation sites are preferentially repressed in mitosis, resulting in pervasive changes in the translation of thousands of start sites and their corresponding protein products. This increased stringency of start codon selection during mitosis results from increased associations between the 40S ribosome and the key regulator of start codon selection, eIF1. We find that increased eIF1-40S ribosome interactions during mitosis are mediated by the release of a nuclear pool of eIF1 upon nuclear envelope breakdown. Selectively depleting the nuclear pool of eIF1 eliminates the changes to translational stringency during mitosis, resulting in the altered synthesis of thousands of protein isoforms. In addition, preventing mitotic translational rewiring results in substantially increased cell death and decreased mitotic slippage in cells that experience a mitotic delay induced by anti-mitotic chemotherapeutics. Thus, cells globally control translation initiation stringency with critical roles during the mammalian cell cycle to preserve mitotic cell physiology. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:52:04.877.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD054183 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jimmy Ly |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-24 08:09:40 | ID requested | |
⏵ 1 | 2024-10-07 10:52:05 | announced | |
2 | 2024-10-22 07:00:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1101/2024.04.06.588385; |
Ly J, Xiang K, Su KC, Sissoko GB, Bartel DP, Cheeseman IM, Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology. bioRxiv, ():(2024) [pubmed] |
10.6019/PXD054183; |
Keyword List
submitter keyword: Translation, ribosomes, mitosis, cell cycle |
Contact List
Iain Cheeseman |
contact affiliation | Whitehead Institute and MIT |
contact email | icheese@wi.mit.edu |
lab head | |
Jimmy Ly |
contact affiliation | PhD student |
contact email | jimmyly@wi.mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054183
- Label: PRIDE project
- Name: Nuclear release of eIF1 globally increases stringency of start codon selection to preserve mitotic arrest physiology