PXD054173-1

PXD054173 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	LINE-1 ORF1p co-immunoprecipitations analyzed by DDA and two DIA methods
Description	Using an anti-ORF1 monoclonal (clone 4H1; Milipore), four co-immunoprecipitations to enrich for LINE-1 ORF1p and its interactors as described in Di Stefano, L.H. et al. (https://doi.org/10.1007/978-1-0716-2883-6_12), from either HEK-293TLD (cells transfected with pMT646- containing a L1 sequence) or N2102Ep (embryonal carcinoma cells with endogenous expression of LINE-1) lysates were then analyzed in DDA, HRMS1, and Variable Window modes. Mouse polyclonal IgG were used as mock IPs as nonspecific binding controls. MMTS alkylated the cysteines. DDA and Variable Window experiments collected spectra for 60 minutes of the one hour run time. The HRMS1 experiments collected spectra for 90 minutes of a two hour run time. The UP000005640 reference proteome for Homo sapiens, including unreviewed sequences and reviewed isoforms was used as a search space (104,558 sequences)
HostingRepository	MassIVE
AnnounceDate	2024-09-10
AnnouncementXML	Submission_2024-09-10_03:03:08.958.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David L. Tabb
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	L-cysteine methyl disulfide; Oxidation
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-24 02:39:33	ID requested
⏵ 1	2024-09-10 03:03:09	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: co-immunoprecipitation, data-independent acquisition, LINE-1

submitter keyword: co-immunoprecipitation, data-independent acquisition, LINE-1

Contact List

John P. LaCava
contact affiliation	University of Groningen
contact email	jlacava@rockefeller.edu
lab head
David L. Tabb
contact affiliation	University of Groningen
contact email	d.l.tabb@rug.nl
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095427/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095427/