PXD054139-1

PXD054139 is an original dataset announced via ProteomeXchange.

Title	Malaria Parasites Hijack Immune Host Splicing Machinery Using Decoy RNAs
Description	Malaria, the most serious mosquito-borne parasitic disease, is mainly caused by the lethal parasite Plasmodium falciparum (Pf), which grows within human red blood cells (RBCs). This master of survival is known for utilizing sophisticated defense strategies to alter the host's immune response. Here, we discovered a new Pf virulence mechanism: the parasite delivers three of its own mRNAs, which belong to the ETRAMP family, to human immune system monocytes, where they are rapidly imported into the host cell nucleus. To further determine whether these parasitic transcripts specifically bind to host proteins in the nucleus, we established an RNA antisense purification (RAP) assay. Remarkably, we identified two host nuclear proteins that are strongly bound by Pf mRNAs: ACIN1 (Acinus) and PNN (Pinin). These two proteins play an important role in host mRNA maturation and splicing and are associated with the exon junction complex. The interaction of Pf mRNAs with these nuclear proteins first impedes the translation of target host proteins, initiating a decoy mechanism, and ultimately leads to a shift in the host immune response towards a pro-inflammatory state. This virulence-promoting strategy represents a new layer of survival defense by the deadliest malaria parasite.
HostingRepository	PRIDE
AnnounceDate	2025-12-17
AnnouncementXML	Submission_2025-12-17_07:15:20.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-23 04:17:45	ID requested
⏵ 1	2025-12-17 07:15:20	announced

Dataset with its publication pending

submitter keyword: Plasmodium falciparum,virulence, Decoy RNAs

Neta Regev-Rudzki
contact affiliation	Faculty of Biochemistry, Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel
contact email	neta.regev-rudzki@weizmann.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054139

PRIDE project URI

• PRIDE
  1. PXD054139
     1. Label: PRIDE project
     2. Name: Malaria Parasites Hijack Immune Host Splicing Machinery Using Decoy RNAs