PXD054127-1

PXD054127 is an original dataset announced via ProteomeXchange.

Title	Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)
Description	In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-DTB) in cell lysates and live cells. We employ label free quantification and data independent acquisition (DIA) on an Evosep One – Bruker timsTOF Pro. The platform was used to screen a library of 80 Cys-reactive covalent fragments in HEK293T and Jurkat cell lysate, identifying functionally relevant hit fragments for numerous cysteine sites and demonstrating the utility of the approach in expanding the ligandable proteome. We have also validated a number of selective interactions through dose response and further expanded our proteomics platform to perform hit expansion studies and in-cell validation.
HostingRepository	PRIDE
AnnounceDate	2024-11-18
AnnouncementXML	Submission_2024-11-18_10:34:05.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	George Biggs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-22 19:34:02	ID requested
⏵ 1	2024-11-18 10:34:06	announced

Dataset with its publication pending

submitter keyword: Chemoproteomics, data independant acquisition, label-free qauntification

Jacob Bush
contact affiliation	Crick-GSK LinkLabs, UK
contact email	jacob.x.bush@gsk.com
lab head
George Biggs
contact affiliation	The Francis Crick Institute
contact email	george.biggs@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054127
     1. Label: PRIDE project
     2. Name: Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)