PXD054061-1

PXD054061 is an original dataset announced via ProteomeXchange.

Title	Co-immunoprecipitation (Co-IP) in Nicotiana benthamiana followed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)
Description	Ralstonia solanacearum species complex causes bacterial wilt in variety crops. Tomato cultivar Hawaii 7996 is a widely used resistance resource, however, the resistance is evaded by virulent strains with underlying mechanisms still unknown. Here, we reported that phylotype II strain ES5-1 can overcome Hawaii 7996 resistance. RipV2, a type Ⅲ effector specifically to phylotype Ⅱ strains, is vital in overcoming tomato resistance. RipV2, which encodes a novel E3 ubiquitin ligase, suppresses immune responses and TNL-mediated cell death. Tomato helper NLR N requirement gene 1 (NRG1), enhanced disease susceptibility 1 (EDS1), and senescence-associated gene 101b (SAG101b) were identified as RipV2 target proteins. RipV2 is essential for ES5-1 virulence in Hawaii 7996 but not in SlNRG1-silenced tomato, demonstrating SlNRG1 to be the bona fide RipV2 virulence target. Our results dissected the mechanisms of RipV2 in disrupting immunity and highlighted the importance of converged immune components in conferring bacterial wiltresistance.
HostingRepository	PRIDE
AnnounceDate	2024-07-20
AnnouncementXML	Submission_2024-07-19_18:40:57.345.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi peipei
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-19 07:17:03	ID requested
⏵ 1	2024-07-19 18:40:57	announced

Dataset with its publication pending

submitter keyword: Nicotiana benthamiana, IP-MS/MS

Li Bo
contact affiliation	The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China
contact email	boli@mail.hzau.edu.cn
lab head
Qi peipei
contact affiliation	State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei
contact email	10152@webmail.hzau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD054061

PRIDE project URI

• PRIDE
  1. PXD054061
     1. Label: PRIDE project
     2. Name: Co-immunoprecipitation (Co-IP) in Nicotiana benthamiana followed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)