PXD054017 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization |
| Description | Complexes of synaptic adhesion molecules instruct the formation, functional specification and plasticity of neuronal synapses. Proteomic and candidate gene studies have identified an array of synaptic adhesion molecules that may cooperate or provide independent columns connecting synaptic compartments, thereby, promoting the nucleation of presynaptic active zones and recruitment of postsynaptic neurotransmitter receptors. Here, we used a systematic large-scale multi-epitope affinity-purification approach (total of >120 purifications with 30 target proteins), combined with quantitative mass spectrometry to comprehensively map trans-synaptic protein networks in the mouse brain. We discover a universal presynaptic core-module consisting of the neurexin proteins and LAR-type receptor protein tyrosine phosphatases (PTPRD,S,F), linked by the tetraspanin proteins T178A, B. These ternary Neurexin-T178-PTPR complexes exclusively form for trans-membrane neurexin isoforms and assemble during biogenesis in the ER. Loss of T178B results in module dissociation and loss of LAR-PTPRs. At synapses, the Neurexin-T178-PTPR module recruits stable trans-synaptic protein networks with specific pre- and post-synaptic partners and secreted extracellular linkers. These networks encompass stable associations with unique postsynaptic GABAergic and glutamatergic neurotransmitter receptor complexes, identifying the Neurexin-T178-PTPR module as a central, universal integrator of trans-synaptic signaling in the central nervous system. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-24 |
| AnnouncementXML | Submission_2026-07-24_10:19:33.360.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD054017 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Alexander Haupt |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF; Q Exactive; LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-07-17 08:59:30 | ID requested | |
| ⏵ 1 | 2026-07-24 10:19:33 | announced | |
Publication List
Keyword List
| submitter keyword: mouse, synapse, neuron, brain, AP-MS. MS |
Contact List
| Prof. Dr. Bernd Fakler |
|---|
| contact affiliation | Institute of Physiology II, Universität Freiburg, Germany |
| contact email | bernd.fakler@physiologie.uni-freiburg.de |
| lab head | |
| Alexander Haupt |
|---|
| contact affiliation | University of Freiburg, Germany |
| contact email | alexander.haupt@physiologie.uni-freiburg.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD054017 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054017
- Label: PRIDE project
- Name: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
