PXD054010-1

PXD054010 is an original dataset announced via ProteomeXchange.

Title	Identification of deubiquitinases controlled by extracellular matrix stiffness in cancer cells
Description	The project was aimed at identifying deubiquitinases (DUBs) involved in cancer cell mechanosignaling. Lysates from cancer cells grown on collagen matrices with varying stiffnesses were incubated with an activity-based HA-tagged ubiquitin probe that covalently binds DUB active site. To identify DUBs differentially activated on soft and stiff collagen hydrogels, lysates were immunoprecipitated with anti-HA agarose beads. Immunoprecipitates were further identified by LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2025-08-22
AnnouncementXML	Submission_2025-08-22_13:59:08.609.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AUDEBERT Stephane
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-17 05:59:26	ID requested
⏵ 1	2025-08-22 13:59:08	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, immunopurification, DUB ABP, ECM stiffness

DECKERT Marcel, PhD
contact affiliation	Team Microenvironment, Signalling and Cancer Inserm UMR1065/C3M, 151 Route de Ginestière BP2 3194, F-06204 Nice cedex 3.
contact email	deckert@unice.fr
lab head
AUDEBERT Stephane
contact affiliation	Marseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact email	stephane.audebert@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD054010

PRIDE project URI

• PRIDE
  1. PXD054010
     1. Label: PRIDE project
     2. Name: Identification of deubiquitinases controlled by extracellular matrix stiffness in cancer cells