PXD054003-1

PXD054003 is an original dataset announced via ProteomeXchange.

Title	The hGID GID4 E3 ubiquitin ligase complex targets ARHGAP11A to regulate cell migration
Description	The human GID (hGID) complex is a key E3 ligase regulating multiple cellular processes, including cell cycle progression and metabolic activity. However, the biological functions controlled by hGID remain poorly investigated. Here, we show that the hGID substrate receptor GID4 regulates cell migration and growth. Biochemical and cellular assays combined with proximity-dependent biotinylation (BioID2) uncovered that GID4 targets ARHGAP11A for degradation. Knockdown of GID4 or inhibition of the GID4 substrate binding pocket impairs motility and directed cell movement, whereas abrogation of ARHGAP11A significantly rescues this cell migration defect. GID4 inhibits cell migration by degrading ARHGAP11A thereby preventing its accumulation at the cell periphery where it turns off RhoA activity. Moreover, our BioID2-GID4 screens revealed a variety of substrate profiles beyond Pro/N-degron motifs with functions in microtubule binding, cytoskeletal dynamics and other biological processes. Overall, we identified cellular functions and a wide range of physiological hGID degradation substrates, which paves the way for deciphering molecular pathways regulated by hGID E3 ligase activity through its GID4 substrate receptor.
HostingRepository	PRIDE
AnnounceDate	2024-09-24
AnnouncementXML	Submission_2024-09-24_04:29:39.187.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Halil Bagci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-17 03:22:30	ID requested
⏵ 1	2024-09-24 04:29:39	announced

Dataset with its publication pending

submitter keyword: E3 Ligase, RhoA, ARHGAP11A,GID, Cell migration, Ubiquitin

Matthias Peter
contact affiliation	Institute of Biochemistry (IBC), ETH Zurich, Switzerland
contact email	matthias.peter@bc.biol.ethz.ch
lab head
Halil Bagci
contact affiliation	ETH Zurich
contact email	halil.bagci@mail.mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD054003

PRIDE project URI

• PRIDE
  1. PXD054003
     1. Label: PRIDE project
     2. Name: The hGID GID4 E3 ubiquitin ligase complex targets ARHGAP11A to regulate cell migration