PXD053960-2

PXD053960 is an original dataset announced via ProteomeXchange.

Title	Oligodendroglial fatty acid metabolism as a central nervous system energy reserve
Description	Brain function requires a constant supply of glucose. However, the brain has no known energy stores, except for glycogen granules in astrocytes. Here, we addressed the question whether oligodendroglial lipid metabolism can provide an energy reserve in white matter tracts. Among other approaches including microscopy and electrophysiology applied to acutely isolated optic nerves from young adult mice used as a model system, we also employed quantitative proteome analysis to study alterations of the whole optic nerve proteome upon glucose deprivation. We compared optic nerve fractions from two different low-glucose incubation conditions (16 h in 0 mM glucose/10 mM sucrose or 24 h in 1 mM glucose/9 mM sucrose) with those from the respective control conditions in regular medium (10 mM glucose/0 mM sucrose). Optic nerve fractions from five animals per condition were processed with replicate digestion, resulting in two technical replicates per biological replicate and thus in a total of 20 LC-MS runs to be compared per individual experiment. We utilized a data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) and an ion mobility-enhanced version thereof (referred to as UDMSE) to achieve both, a correct quantification of exceptionally abundant myelin proteins and a comprehensive coverage of the optic nerve proteome. Label-free protein quantification revealed a non-significant trend toward higher myelin protein abundance, a reduced abundance of some glycolytic enzymes, an increased abundance of fatty acid binding proteins and enzymes of fatty acid metabolism, as well as a higher steady-state level of some autophagy-related proteins. Taken together, our study indicates that ongoing oligodendroglial lipid degradation feeds rapidly into white matter energy metabolism, and that the imbalance of myelin synthesis and degradation can underlie unexplained myelin thinning in aging and disease.
HostingRepository	PRIDE
AnnounceDate	2024-08-27
AnnouncementXML	Submission_2024-08-27_03:38:39.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-15 16:33:57	ID requested
1	2024-07-16 12:12:12	announced
⏵ 2	2024-08-27 03:38:39	announced	2024-08-27: Updated project metadata.

10.1038/S41593-024-01749-6;

submitter keyword: label-free protein quantification, lipid metabolism, mass spectrometry, myelin, whole optic nerve proteome,optic nerve, oligodendrocytes

Prof. Dr. Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@mpinat.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053960
     1. Label: PRIDE project
     2. Name: Oligodendroglial fatty acid metabolism as a central nervous system energy reserve