PXD053944-1

PXD053944 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation of mitochondrial ribosome complex of Toxoplasma gondii
Description	Mitochondrial ribosomes in Apicomplexan parasite Toxoplasma gondii are expected to be divergent from all the other known mitoribosomes owing to the extremely fragmented and reduced rRNAs. To elucidate the structure and composition of these mitoribosomes, we performed immunoprecipitation of the FLAG epitope tagged bL12m subunit of mitoribosome using anti-FLAG M2 agarose beads. The elute was used to freeze CryoEM grids for single particle analysis and bound beads were subjected to Liquid chromatography–mass spectrometry (LC–MS) to analyse the co-immunoprecipitated proteins.
HostingRepository	PRIDE
AnnounceDate	2025-04-21
AnnouncementXML	Submission_2025-04-21_09:56:00.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shikha Shikha
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-15 02:09:41	ID requested
⏵ 1	2025-04-21 09:56:02	announced

10.1038/s41467-025-56057-9;

Shikha S, Tobiasson V, Ferreira Silva M, Ovciarikova J, Beraldi D, M, ü, hleip A, Sheiner L, Numerous rRNA molecules form the apicomplexan mitoribosome via repurposed protein and RNA elements. Nat Commun, 16(1):817(2025) [pubmed]

submitter keyword: Toxoplasma gondii,Mitoribosome

Lilach Sheiner
contact affiliation	School of Infection and Immunity ,MVLS, University of Glasgow
contact email	lilach.sheiner@glasgow.ac.uk
lab head
Shikha Shikha
contact affiliation	University of Glasgow
contact email	shikha.shikha@glasgow.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053944

PRIDE project URI

• PRIDE
  1. PXD053944
     1. Label: PRIDE project
     2. Name: Immunoprecipitation of mitochondrial ribosome complex of Toxoplasma gondii