PXD053940-1

PXD053940 is an original dataset announced via ProteomeXchange.

Title	Uncover proteins binding HBV rcDNA
Description	To comprehensively uncover host proteins involved in HBV rcDNA repair, we employed a rcDNA repair system, which can simulate the process of cccDNA formation in vitro. Biotinylated rcDNA first bonds with streptavidin magnetic beads and is then incubated with cell extracts of HepG2-NTCP-K7 cells to facilitate rcDNA repair. The proteins recruited by biotinylated rcDNA were identified by mass spectrometry. Proteins with the number of captured peptides by biotinylated HBV rcDNA was greater than or equal to 5 and twice that of control were classified as the target proteins. Based on this, a total of 83 target proteins were screened to perform the GeneOntology (GO) analysis, revealing that the top-listed genes were involved in DNA repair.
HostingRepository	PRIDE
AnnounceDate	2024-12-18
AnnouncementXML	Submission_2024-12-18_04:14:29.878.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaitao Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-14 23:58:31	ID requested
⏵ 1	2024-12-18 04:14:30	announced

Dataset with its publication pending

submitter keyword: HBV rcDNA, DNA repair,hepatocytes

Kaitao Zhao
contact affiliation	State Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy and Immunology, Institute of Medical Virology, TaiKang Medical School, Wuhan University, Wuhan, 430071, China
contact email	00032927@whu.edu.cn
lab head
Kaitao Zhao
contact affiliation	Wuhan University
contact email	00032927@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD053940

PRIDE project URI

• PRIDE
  1. PXD053940
     1. Label: PRIDE project
     2. Name: Uncover proteins binding HBV rcDNA