PXD053920-1

PXD053920 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics and Phosphoproteomics Profiling of Meiotic Cell Divisions in the Fission Yeast Schizosaccharomyces pombe
Description	In eukaryotes, chromosomal DNA is equally distributed to daughter cells during mitosis, whereas the number of chromosomes is halved during meiosis. Despite considerable progress in understanding the molecular mechanisms that regulate mitosis, there is currently a lack of complete understanding of the molecular mechanisms regulating meiosis. Here, we took advantage of the fission yeast Schizosaccharomyces pombe, for which highly synchronous meiosis can be induced, and performed quantitative proteomics and phosphoproteomics analyses to track changes in protein expression and phosphorylation during meiotic cell divisions. We compared the proteomes and phosphoproteomes of mitotic cells with cells harvested around meiosis I, or meiosis II in strains bearing either the temperature-sensitive pat1-114 allele or conditional ATP analog-sensitive pat1-as2 allele of the Pat1 kinase. Comparing pat1-114 with pat1-as2 also allowed us to investigate the impact of elevated temperature (25°C versus 34°C) on meiosis, an issue that sexually reproducing organisms face due to climate change. Using TMTpro 18plex labeling and phosphopeptide enrichment strategies, we performed quantification of a total of 4673 proteins and 7172 phosphosites in S. pombe. We found that the protein level of 2680 proteins and the rate of phosphorylation of 4005 phosphosites significantly changed during progression of S. pombe cells through meiosis. The proteins exhibiting changes in expression and phosphorylation during meiotic cell divisions were represented mainly by those involved in the meiotic cell cycle, meiotic recombination, meiotic nuclear division, meiosis I, centromere clustering, microtubule cytoskeleton organization, ascospore formation, organonitrogen compound biosynthetic process, carboxylic acid metabolic process, gene expression, and ncRNA processing, among others. In summary, our findings provide global overview of changes in the levels and phosphorylation of proteins during progression of S. pombe cells through meiosis at normal and elevated temperatures, laying the groundwork for further elucidation of the functions and importance of specific proteins and their phosphorylation in regulating meiotic cell divisions in this yeast.
HostingRepository	PRIDE
AnnounceDate	2024-10-09
AnnouncementXML	Submission_2024-10-09_06:15:52.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barath
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 10:01:18	ID requested
⏵ 1	2024-10-09 06:15:57	announced
2	2024-10-22 07:01:00	announced	2024-10-22: Updated project metadata.

Sivakova B, Wagner A, Kretova M, Jakubikova J, Gregan J, Kratochwill K, Barath P, Cipak L, Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe. Sci Rep, 14(1):23105(2024) [pubmed]

10.1038/s41598-024-74523-0;

submitter keyword: phosphoproteome,Schizosaccharomyces pombe, meiosis, Pat1 protein kinase

Dr Klaus Kratochwill
contact affiliation	Medical University of Vienna
contact email	klaus.kratochwill@meduniwien.ac.at
lab head
Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053920
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics and Phosphoproteomics Profiling of Meiotic Cell Divisions in the Fission Yeast Schizosaccharomyces pombe