PXD053906-1

PXD053906 is an original dataset announced via ProteomeXchange.

Title	Increased viral tolerance mediates by antiviral RNA interference in bat cells
Description	Bats harbor highly virulent viruses that can infect other mammals, including humans, posing questions about their immune tolerance mechanisms. Bat cells employ multiple strategies to limit virus replication and virus-induced immunopathology, but the coexistence of bats and fatal viruses remains poorly understood. Here, we investigated the antiviral RNA interference (RNAi) pathway in bat cells and discovered that they have an enhanced antiviral RNAi response, producing canonical viral small interfering RNAs (vsiRNAs) upon Sindbis virus (SINV) infection that were missing in human cells. Disruption of Dicer function resulted in increased viral load for three different RNA viruses in bat cells, indicating an interferon-independent antiviral pathway. Furthermore, our findings reveal the simultaneous engagement of Dicer and pattern-recognition receptors (PRRs), such as retinoic acid-inducible gene I (RIG-I), with double-stranded RNA, suggesting that Dicer attenuates the interferon response initiation in bat cells. These insights advance our comprehension of the distinctive strategies bats employ to coexist with viruses.
HostingRepository	PRIDE
AnnounceDate	2024-07-12
AnnouncementXML	Submission_2024-07-12_02:59:56.327.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunpeng Dai
SpeciesList	scientific name: Pteropus alecto (Black flying fox); NCBI TaxID: 9402;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 01:12:31	ID requested
⏵ 1	2024-07-12 02:59:56	announced

Dataset with its publication pending

submitter keyword: Pteropus alecto, SINV, dsRNA-IP, J2-IP

Yang Li
contact affiliation	State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University
contact email	yangli15@fudan.edu.cn
lab head
Yunpeng Dai
contact affiliation	Fudan University
contact email	ypdai20@fudan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053906
     1. Label: PRIDE project
     2. Name: Increased viral tolerance mediates by antiviral RNA interference in bat cells