PXD053902-1

PXD053902 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomic and global phosphoproteomic analysis of cisplatin-induced apoptosis in A549 cells
Description	Protein phosphorylation, a widely occurring and significant post-translational modification, is integral to various biological processes. We previously utilized a protein affinity probe to identify genes damaged by cisplatin, revealing that it inflicts substantial damage on protein kinase and protein phosphatase genes. In this study, we investigated cisplatin-induced alterations in the proteome and global phosphoproteome of A549 cells. Employing Fe-IMAC beads and tyrosine phosphorylation enrichment antibodies, we identified 6944 protein groups and 18,274 phosphorylation sites on 4,915 proteins across three biological replicates of both cisplatin-treated A549 cells and control cells. Among these, 730 tyrosine phosphorylation sites were identified—marking the most substantial discovery of such sites in A549 cells following cisplatin treatment. Bioinformatics analysis indicated that the proteins exhibiting significant phosphorylation level changes, which are predominantly involved in RNA processing, modification, transcription, translation, and the spliceosome. This suggests that cisplatin-induced damage to protein kinases and phosphatases may disrupt the normal function of these proteins, consequently impairing DNA replication, RNA translation, and shearing, ultimately culminating in tumor cell death. Moreover, we cross-referenced our proteomic data with our previously obtained cisplatin-damaged genes, observing that the majority of down-regulated proteins derived from cisplatin-induced gene damage.
HostingRepository	PRIDE
AnnounceDate	2024-10-20
AnnouncementXML	Submission_2024-10-20_05:47:29.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	luyu qi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-11 21:07:17	ID requested
⏵ 1	2024-10-20 05:47:30	announced

10.1016/J.BBRC.2024.150846;

submitter keyword: phosphoproteomic, cisplatin-damage genes,proteomic, cisplatin, A549 cells

Jianzhong Shao
contact affiliation	College of Life Sciences, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Zhejiang University, Hangzhou, China
contact email	shaojz@zju.edu.cn
lab head
luyu qi
contact affiliation	Zhejiang University
contact email	luyuqi1993@foxmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053902
     1. Label: PRIDE project
     2. Name: Integrated proteomic and global phosphoproteomic analysis of cisplatin-induced apoptosis in A549 cells