PXD053891-1

PXD053891 is an original dataset announced via ProteomeXchange.

Title	The identification of biological stains at crime scene: a promising role for proteomics and machine learning
Description	Identification of stains in criminalistics remains a critical challenge in forensic sciences. In particular, determining the biological origin of a trace can be crucial in an investigation in order to understand the circumstances in which it was deposited. This study aims to develop three distinct tests based on proteomics data for identifying human bodily fluids. The first test focuses on constructing specific peptide biomarkers for each fluid. The second test quantitatively assesses ratios between peptide abundances. The final test employs artificial intelligence, utilizing multioutput Random Forest, to distinguish between pure fluids and mixtures. Data from 20 individuals per fluid and 78 mixtures are analyzed. This research underscores the potential of proteomics in forensic bodily fluid identification. The evaluation of the different tests highlights their complementarity, as they focus on different features obtained from a single experiment.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_12:20:42.114.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Rosenberg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-11 15:00:26	ID requested
⏵ 1	2025-10-06 12:20:42	announced

10.1021/ACS.ANALCHEM.5C01795;

submitter keyword: Human, vaginal fluid, urine, body fluids identification, blood, saliva, semen, LC-MS/MS, forensic

Joëlle Vinh
contact affiliation	Spectrométrie de Masse Biologique et Protéomique, CNRS, UMR 8249, Rue Vauquelin, 75005, Paris, France
contact email	joelle.vinh@espci.fr
lab head
Anna Rosenberg
contact affiliation	Department of biology, ENS Paris-Saclay SMBP (Spectrométrie de Masse Biologique et Protéomique), ESPCI-PSL, CNRS UMR 8249
contact email	anna.rosenberg@ens-paris-saclay.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD053891

PRIDE project URI

• PRIDE
  1. PXD053891
     1. Label: PRIDE project
     2. Name: The identification of biological stains at crime scene: a promising role for proteomics and machine learning