PXD053868-1

PXD053868 is an original dataset announced via ProteomeXchange.

Title	Disulfide Bond Shuffling Induces Molecular Crosstalk: Delayed Insulin Aggregation and Enhanced Neurotoxicity
Description	Our study explores the novel concept of disulfide bond shuffling-provoked oligomer crosstalk in insulin. We demonstrate that this dynamic process not only modulates the aggregation kinetics of insulin but also significantly influences its cytotoxic potential. Through a combination of biophysical techniques and cellular assays, we elucidate the molecular mechanisms underlying this phenomenon. Our findings reveal that disulfide bond shuffling in insulin oligomers can lead to the formation of novel, metastable species. These species, in turn, engage in crosstalk with other oligomers, thereby delaying the overall aggregation process. Intriguingly, our data also suggest that these metastable oligomers exhibit enhanced cytotoxicity compared to their fully aggregated counterparts. This observation underscores the potential biological implications of disulfide bond shuffling in insulin and opens up new avenues for investigating the role of protein misfolding and aggregation in disease pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-07-23
AnnouncementXML	Submission_2025-07-23_08:46:24.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gongyu Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-10 19:14:41	ID requested
⏵ 1	2025-07-23 08:46:25	announced

10.1038/S41467-025-62257-0;

submitter keyword: disulfide, neurotoxicity, ion mobility-mass spectrometry, disulfide bond shuffling, insulin

Gongyu Li
contact affiliation	Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Science, Frontiers Science Center for New Organic Matter, College of Chemistry, Nankai University, Tianjin 300071, China
contact email	ligongyu@nankai.edu.cn
lab head
Gongyu Li
contact affiliation	Nankai University
contact email	ligongyu@nankai.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD053868

PRIDE project URI

• PRIDE
  1. PXD053868
     1. Label: PRIDE project
     2. Name: Disulfide Bond Shuffling Induces Molecular Crosstalk: Delayed Insulin Aggregation and Enhanced Neurotoxicity