PXD053803-1

PXD053803 is an original dataset announced via ProteomeXchange.

Title	Neurodevelopmental cellular changes in rodents after 900 MHz radiofrequency exposureNeurodevelopmental cellular changes in rodents after 900 MHz radiofrequency exposure
Description	This study investigates the effects of non-thermal levels of 900 MHz radiofrequency electromagnetic field (RF-EMF) which is used in mobile phone technology on neurodevelopment and neural stem cell differentiation. In vivo in the rat, the effects of pre- and post-natal 0.08 and 0.4 W/kg exposure were assessed on the proteomic profile at PND postnatal day 0 (PND) and on proliferation, synaptogenesis, and oxidative stress in the hippocampus and primary motor cortex of rats at PND 8 and PND 17. Complementary Mechanistic endpoints on cell differentiation were studied in vitro using stem cells exposed to 900 MHz RF-EMF at a non-thermal level for 3 or 7 days in vitro at the lowest SAR (0.08 W/kg). Our in vivo results showed a decrease in BDNF level and cells proliferation with a decrease in synapses balance. Increased proliferation, apoptosis, and double-strand DNA breaks in neural stem cells (NSCs) were also observed. Smaller ratio of B1 cells and a higher ratio of oligodendrocyte progenitor cells and astrocytes were observed in the exposed NSPCs. These findings suggest that developmental RF-EMF exposure induce changes in neurodevelopment in vivo from PND 0 and until PND 17 specifically on cellular proliferation supported by alterations in NSPCs in vitro, leading to the hypothesis of a vulnerability of developing central nervous system towards RF-EMF exposures at regulatory thresholds.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_07:11:35.552.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053803
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paulo MARCELO
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 08:48:41	ID requested
⏵ 1	2025-09-11 07:11:37	announced

10.1016/j.neuro.2025.103312;

10.6019/PXD053803;

Bodin R, Godin L, Mougin C, Lecomte A, Larrigaldie V, Feat-Vetel J, M, é, resse S, Mont, é, cot-Dubourg C, Marcelo P, Mortaud S, Villegier AS, MHz radiofrequency exposure. Neurotoxicology, 111():103312(2025) [pubmed]

submitter keyword: Radiofrequency fields

Proliferation

Synaptogenesis

Oxidative stress

Neuroproteomics

Neurodevelopment

Neurotoxicology

MARCELO Paulo
contact affiliation	Universite Picardie Jules Verne
contact email	paulo.marcelo@u-picardie.fr
lab head
Paulo MARCELO
contact affiliation	Université de Picardie Jules Verne
contact email	paulo.marcelo@u-picardie.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053803
     1. Label: PRIDE project
     2. Name: Neurodevelopmental cellular changes in rodents after 900 MHz radiofrequency exposureNeurodevelopmental cellular changes in rodents after 900 MHz radiofrequency exposure