PXD053775-1

PXD053775 is an original dataset announced via ProteomeXchange.

Title	Proteomic Characterization of Cardioprotective Human Acellular Amniotic Fluid
Description	Amniotic fluid-derived products are a promising resource for cell therapy and tissue engineering due to their anti-inflammatory, angiogenic, and antifibrotic properties. Human amniotic fluid (hAF) has been used in medical applications such as wound healing, skin disorders, and ophthalmic conditions. Recently, we demonstrated that hAF is an effective treatment for myocardial ischemia-reperfusion injury in adult rats. However, the protein composition of full-term acellular hAF has remained poorly characterized. To uncover the biologically active components underlying hAF’s cardioprotective effects, we conducted a global proteomic analysis of hAF collected from six patients at full-term cesarean sections. Previously shown to improve cardiac function in ischemic rats, these samples were analyzed by using tandem mass spectrometry. We identified 657 proteins, including 148 unique to the deep learning platform Inferys. Bioinformatic analysis revealed that these proteins are involved in immunity, inflammatory responses, cell adhesion, and apoptotic signaling pathways. In addition, these proteins were highly modified, with methylation and deamidation being the most abundant modifications. This study represents the first mass-spectrometry-based characterization of full-term, acellular hAF, suggesting that hAF offers a wide array of immune-modulating proteins working together to provide robust cardioprotection and a valuable treatment for ischemia-reperfusion injury.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_21:08:56.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Bia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 16:00:23	ID requested
⏵ 1	2025-05-06 21:08:57	announced

10.1021/acsomega.4c09451;

Bia R, Mitchell G, Javan H, Nickel I, Pierce J, Selzman CH, Franklin S, Proteomic Characterization of Cardioprotective Human Acellular Amniotic Fluid. ACS Omega, 10(7):6918-6926(2025) [pubmed]

submitter keyword: Human, Amniotic Fluid, Proteomics, LC-MS/MS

Sarah Franklin
contact affiliation	University of Utah, Salt Lake City, Utah, United States
contact email	sarah.frank@utah.edu
lab head
Ryan Bia
contact affiliation	University of Utah
contact email	ryan.bia@utah.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053775

PRIDE project URI

• PRIDE
  1. PXD053775
     1. Label: PRIDE project
     2. Name: Proteomic Characterization of Cardioprotective Human Acellular Amniotic Fluid