PXD053760-1

PXD053760 is an original dataset announced via ProteomeXchange.

Title	ARL3 GTPases facilitate ODA16 unloading from IFT in motile cilia
Description	Eukaryotic cilia and flagella are essential for cell motility and sensory functions. Their biogenesis and maintenance rely on the intraflagellar transport (IFT). Several cargo adapters have been identified to aid IFT cargo transport, but how ciliary cargos are discharged from IFT remains largely unknown. During our explorations of small GTPases ARL13 and ARL3 in Trypanosoma brucei, we found that ODA16, a known IFT cargo adapter found exclusively in motile cilia, is a specific effector of ARL3. To investigate how TbARL3A and TbARL3C regulate TbODA16, we performed proximity-dependent biotin identification (BioID) and mass spectrometry analyses for TbODA16 in the presence or absence of TbARL3A and TbARL3C.
HostingRepository	PRIDE
AnnounceDate	2024-09-05
AnnouncementXML	Submission_2024-09-04_20:18:12.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yameng Huang
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	biotinylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 08:08:41	ID requested
⏵ 1	2024-09-04 20:18:12	announced

10.1126/SCIADV.ADQ2950;

submitter keyword: TbODA16 BioID2

Cynthia Y. He
contact affiliation	Department of Biological Sciences, National University of Singapore
contact email	dbshyc@nus.edu.sg
lab head
Yameng Huang
contact affiliation	Department of Biological Sciences, National University of Singapore
contact email	yameng.h@nus.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053760
     1. Label: PRIDE project
     2. Name: ARL3 GTPases facilitate ODA16 unloading from IFT in motile cilia