PXD053753-1

PXD053753 is an original dataset announced via ProteomeXchange.

Title	Absolute quantification analysis of ubiquitin chains attached to Nrf1 purified from NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2.
Description	We examined ubiquitin-linkages on Nrf1 purified from cell lysates of NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2. The ubiquitin linkage types were quantified using the LC-MS/MS-based absolute quantification method, ubiquitin parallel reaction monitoring (Ub-AQUA/PRM).
HostingRepository	PRIDE
AnnounceDate	2024-08-06
AnnouncementXML	Submission_2024-08-06_01:04:33.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hikaru Tsuchiya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 05:26:03	ID requested
⏵ 1	2024-08-06 01:04:34	announced
2	2024-10-22 06:52:28	announced	2024-10-22: Updated project metadata.

10.1016/J.MOLCEL.2024.07.013;

submitter keyword: Nrf1, ubiquitination,FBS2

Yukiko Yoshida
contact affiliation	Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science
contact email	yoshida-yk@igakuken.or.jp
lab head
Hikaru Tsuchiya
contact affiliation	okyo Metropolitan Institute of Medical Science
contact email	tsuchiya-hk@igakuken.or.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD053753

PRIDE project URI

• PRIDE
  1. PXD053753
     1. Label: PRIDE project
     2. Name: Absolute quantification analysis of ubiquitin chains attached to Nrf1 purified from NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2.