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PXD053738-1

PXD053738 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Redox proteomic analysis of H2O2 -treated Jurkat cells and effects of bicarbonate and peroxiredoxins 1 and 2
Description	Oxidation of thiol proteins and redox signaling occur in cells exposed to H2O2 but mechanisms are unclear. We used redox proteomics to seek evidence of oxidation of specific proteins either by a mechanism involving reaction of H2O2 with CO2/bicarbonate to give the more reactive peroxymonocarbonate, or via a relay involving peroxiredoxins (Prdx1 or Prdx2). We measured changes in oxidation state of specific Cys-SH residues on treating Jurkat T cells with H2O2,by isotopically labeling reduced thiols and analyzing the tagged peptides by mass spectrometry. Cells treated in the absence and presence of bicarbonate were compared, as well as wildtype cells and cells in which Prdx1 or Prdx2 had been knocked out.
HostingRepository	iProX
AnnounceDate	2024-07-08
AnnouncementXML	Submission_2024-07-07_21:13:02.836.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Fu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	(13)C labeled residue
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-07 21:09:02	ID requested
⏵ 1	2024-07-07 21:13:03	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: thiol redox proteomic changes

Contact List

Jing Yang
contact affiliation	National Center for Protein Sciences (PHOENIX, Beijing)
contact email	yangjing54@hotmail.com
lab head
Ling Fu
contact affiliation	National Center for Protein Sciences (PHOENIX, Beijing)
contact email	flsmt@163.com
dataset submitter

Full Dataset Link List

iProX dataset URI