PXD053696-1

PXD053696 is an original dataset announced via ProteomeXchange.

Title	Hypoxia-induced adaptations of N-glycomes and proteomes in breast cancer cells and their secreted extracellular vesicles
Description	The hypoxic tumor microenvironment significantly impacts cellular behavior and intercellular communication, with extracellular vesicles (EVs) playing a crucial in promoting angiogenesis, metastasis, and host immunosuppression. Aberrant surface N-glycan expression in EVs is closely associated with cancer progression and metastasis. We hypothesize that hypoxic tumors synthesize specific hypoxia-induced N-glycans, which are packaged into EVs to modulate the tumor microenvironment and drive cancer progression. This study utilized nano-LC-MS/MS to integrate quantitative proteomic and N-glycomic analyses of both cells and EVs derived from the MDA-MB-231 breast cancer cell line, cultured under normoxic and hypoxic conditions. Whole N-glycome and proteome profiling revealed that hypoxia changes the expression of asparagine N-linked glycosylation and glycolysis/gluconeogenesis proteins in cells to altered N-glycosylation for their adaptation to low oxygen conditions. Distinct N-glycan types, high-mannose glycans like Man3 and Man9 were highly abundant in hypoxic cells. On the other hand, alterations in sialylation and fucosylation patterns were observed in hypoxic cells. Furthermore, hypoxia-induced EVs exhibit a signature consisting of mono-antennary structures and specific N-glycans (H4N3F1S2, H3N3F1S0, and H7N4F3S2, H8N4F1S0 and H8N6F1S2), which significantly associated with a poor prognosis for breast tumor, potentially altering interactions within the tumor microenvironment to promote tumorigenesis and metastasis. Our findings provide an overview of N-glycan profiles, particularly under hypoxic conditions, offer insights into potential biomarkers for tracking tumor microenvironment dynamics and for developing precision medicine approaches in oncology.
HostingRepository	PRIDE
AnnounceDate	2024-11-07
AnnouncementXML	Submission_2024-11-07_00:28:12.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bente Siebels
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-05 06:31:44	ID requested
⏵ 1	2024-11-07 00:28:13	announced

10.3390/IJMS251810216;

submitter keyword: N-glycomics, Proteomics,N-glycosylation, Hypoxia, Extracellular vesicles

Prof. Dr. Hartmut Schlueter
contact affiliation	Section for Mass Spectrometry and Proteomics, Center for Diagnsostics, University Medical Center Hamburg-Eppendorf
contact email	hschluet@uke.de
lab head
Bente Siebels
contact affiliation	Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf
contact email	b.siebels@uke.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053696
     1. Label: PRIDE project
     2. Name: Hypoxia-induced adaptations of N-glycomes and proteomes in breast cancer cells and their secreted extracellular vesicles