PXD053673-1

PXD053673 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics analysis for anti-apoptotic signaling driven by CXCL4 oligomerization in MCL cells
Description	To determine the anti-tumor effects induced by interfering CXCR4 receptor oligomerization, we treated the cells with a small molecule and a nanobody in the Z-138 cell line (mantle cell lymphoma, MCL), separately. By using phospho-enrichment method, we identified 15,563 phosphopeptides and quantified more than 15,000 phosphosites. Deep phosphoproteome profiling enables us to characterize the phosphorylation changes upon the disruption of CXCR4 oligomerization on the cell surface. Followed pathway analysis shows that phosphorylation changes in apoptotic regulation were found upon the treatment of drugs. Hence, we speculated that inhibition of CXCR4 oligomerization may sensitive Z-138 cells to apoptosis in combination with another therapeutic trigger.
HostingRepository	PRIDE
AnnounceDate	2024-09-24
AnnouncementXML	Submission_2024-09-24_05:44:55.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziliang Ma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-04 22:50:32	ID requested
⏵ 1	2024-09-24 05:44:55	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomics, CXCR4 oligomerization, apoptosis, LC-MS/MS

wei wu
contact affiliation	1. Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, Immunos, Singapore 138648, Republic of Singapore 2. Department of Pharmacy, National University of Singapore 3. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, the Netherlands
contact email	wu_wei@immunol.a-star.edu.sg
lab head
Ziliang Ma
contact affiliation	Utrecht University
contact email	z.ma@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD053673

PRIDE project URI

• PRIDE
  1. PXD053673
     1. Label: PRIDE project
     2. Name: Phosphoproteomics analysis for anti-apoptotic signaling driven by CXCL4 oligomerization in MCL cells